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Patents/USRE049835

USRE049835No. USRE049835reissueGranted 2/13/2024

Claims (10)

Claim 1 (Independent)

1. A method of determining the alleles present in a DNA sample, the method comprising: obtaining a DNA sample to be analyzed; selecting a set of STR marker loci of the DNA sample to be analyzed that can be amplified together in a multiplex amplification reaction, wherein the set of STR marker loci are selected from the group consisting of: 18-3, 4-2, 6-7, 9-2, 15-3, 6-4, 12-1, 5-5 and X-1; providing a set of oligonucleotide primer pairs, wherein each oligonucleotide primer pair in the set flanks a single locus in the set of STR marker loci, and wherein each oligonucleotide primer pair is capable of amplification of a single locus from the set of STR marker loci in a multiplex amplification reaction; co-amplifying the set of STR marker loci in a multiplex amplification reaction, wherein the product of the multiplex amplification reaction comprises a mixture of amplified alleles from each of the co-amplified loci in the set of STR marker loci; evaluating the products of the co-amplification reaction to determine the alleles present at each of the loci analyzed in the set of STR marker loci within the DNA sample; and wherein the source of the DNA sample to be analyzed is at least one of a mouse and a cell line derived from a mouse.

Show 9 dependent claims
Claim 2 (depends on 1)

2. The method of claim 1 , wherein the mouse is selected from the group consisting of Mus musculus musculus, Mus musculus domesticus, Mus musculus molossinus and Mus musculus castaneus.

Claim 3 (depends on 1)

3. The method of claim 1 , wherein the DNA sample to be analyzed comprises DNA derived from at least two different mouse sources.

Claim 4 (depends on 1)

4. The method of claim 1 , wherein the DNA sample to be analyzed further comprises DNA derived from at least one other source that is a non-mouse source.

Claim 5 (depends on 4)

5. The method of claim 4 , wherein the non-mouse source is at least one of human and African green monkey.

Claim 6 (depends on 5)

6. The method of claim 5 , wherein the set of oligonucleotide primer pairs further comprises an oligonucleotide primer pair configured to amplify an STR allele from the non-mouse source.

Claim 7 (depends on 1)

7. The method of claim 1 , wherein at least one oligonucleotide primer in each oligonucleotide pair comprises a detection label.

Claim 8 (depends on 7)

8. The method of claim 7 , wherein the detection label comprises a fluorescent dye.

Claim 9 (depends on 8)

9. The method of claim 8 , wherein evaluating the products of the co-amplification reaction comprises measuring fluorescence emission upon excitation of the detection label with light.

Claim 10 (depends on 7)

10. The method of claim 7 , comprising using at least four oligonucleotide primer pairs, wherein at least one primer of each oligonucleotide primer pair is labeled with a detection label, and wherein at least four different detection labels are used.

Full Description

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Citations

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