1. A method for heat inducible production of a Rep protein dependent replication origin plasmid vector, comprising: a. cloning the plasmid replication regulating Rep protein into an expression vector to create a P L -Rep protein expression cassette in which said Rep protein is expressed under the control of the P L promoter and said P L promoter further comprises an OL1 mutation in which repressor binding to OL1 is decreased or lost by mutation in OL1, said OL1 mutation comprising either a single base substitution or a single base deletion within the P L promoter (−35 to −10) SEQ ID NO:10; b. integrating said P L -Rep protein expression cassette into a host strain genome to create a P L -Rep protein host strain in which expression from the said P L -Rep protein expression cassette is repressed by a temperature sensitive lambda repressor expressed from the host strain genome; c. transforming said P L -Rep protein host strain with said Rep protein dependent replication origin plasmid vector; d. isolating the resultant transformed bacterial cells; e. propagating said transformed bacterial cells at 25-32° C. to maintain the said Rep protein dependent plasmid vector at a basal copy number; and f. inducing said transformed bacterial cells at 37-42° C. to increase copy number of said Rep protein dependent plasmid vector.

8. A method for heat inducible production of a Rep protein dependent plasmid vector, comprising: a. obtaining bacterial cells transformed with the Rep protein dependent plasmid vector, the bacterial cells comprising a P L promoter and a Rep protein, wherein the Rep protein dependent plasmid vector comprises a replication origin, wherein the P L promoter controls the expression of the Rep protein, wherein the P L promoter comprises the nucleic acid sequence of SEQ ID NO:10, and wherein the P L promoter comprises an OL1 mutation within the nucleic acid sequence of SEQ ID NO: 10, wherein the OL1 mutation comprises either a single base substitution or a single base deletion that decreases or prevents repressor biding to OL1; b. incubating said bacterial cells at 25-32° C. to maintain the Rep protein dependent plasmid vector at a basal copy number; and c. incubating said bacterial cells at 37-42° C. to increase copy number of said Rep protein dependent plasmid vector.

9. The method of claim 8, wherein said OL1 mutation within the nucleic acid sequence of SEQ ID NO:10 is selected from the group consisting of SEQ ID NO: 11 and SEQ ID NO: 12.

10. The method of claim 8, wherein the replication origin is selected from the group consisting of a R6K replication origin, ColE2-P9 replication origin and ColE2 related replication origin.

11. The method of claim 8, wherein the replication origin is a R6K replication origin, and wherein said Rep protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 13 and SEQ ID NO: 14.

12. The method of claim 8, wherein the replication origin is a ColE2-P9 replication origin, and wherein the Rep protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 15 and SEQ ID NO: 16.

13. The method of claim 8, wherein said bacteria cells further comprises a genomically expressed RNA-IN regulated selection marker.

14. The method of claim 8, wherein said Rep protein dependent plasmid vector has a vector backbone with at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 39, SEQ ID NO: 40, and SEQ ID NO: 41.