Cannabis Plant Named ‘PTC8506V’

Genus/species: Cannabis sativa.

Variety denomination: ‘PTC8506V’.

BACKGROUND

Cannabis plants contain over a hundred known cannabinoids, which bind to endogenous endocannabinoid receptors. Varinolic cannabinoids, as known as varins, are a type of cannabinoid compounds having three carbon atoms in their alkyl side chain instead of the five carbon atom alkyl side chains more commonly associated with cannabinoids. Two such varins are tetrahydrocannabivarin (THCV) and cannabidivarin (CBDV), which are homologues of tetrahydrocannabinol (THC) and cannabidiol (CBD), respectively. Each varin has a unique pharmacological profile and distinct molecular targets.

THCV and CBDV have potential benefits across a broad set of applications. Cannabis plants or extracts with high THCV levels, for example, can be used as an agent for anticonvulsant activity, obesity-associated glucose intolerance, appetite suppression, anxiety management for PTSD, diabetic neuropathy, and major neuropathic and pain related pathologies. Another THCV application is that of an appetite suppressing compound. CBDV has been shown to have anti-epileptic and anticonvulsant activity.

Research and development as well as the sale of varin products has been limited due to low commonly occurring levels of varins in Cannabis flower. The ability to produce Cannabis with high varin levels will create a platform for a new Cannabinoid category with differentiated, high margin products in both medical and recreational markets. The present application solves these problems by providing a novel Cannabis plant having high THCV, which is referred to by the variety name ‘PTC8506V’.

SUMMARY

The new variety of Cannabis sativa was created by sourcing Cannabis plants that produce THCV and THC. Crosses were conducted to elevate further THCV, THC and/or other varins. All breeding, cultivation, and chemotype analyses were conducted in Hillsboro, Oregon. ‘PTC8506V’ resulted from the cross of a high THC pollen receiver plant (‘21GAQ-1271’, unpatented) and a high THCV pollen donor plant (‘21VLP5-1-102’, unpatented). ‘21GAQ-1271’ is a clonal variety with a THC content of 22.2%, and a THCV content below the limit of detection (LOD). ‘21GAQ-1271’ has greenish-purple flowers with heavy trichome coverage, moderate density, and strong aroma. ‘21GAQ-1271’ has an upright plant growth habit with moderately long internodes and matures in 8-9 weeks. ‘21VLP5-1-102’ had a THC content of 1.2%, and THCV content of 5.66%. ‘21VLP5-1-102’ flowers are greenish-purple with low trichome coverage, very low density, and moderately weak aroma. ‘21VLP5-1-102’ has a moderately wide plant growth habit with very long internodes and matures in 10-12 weeks. A clone of ‘21VLP5-1-102’ was crossed onto a clone of ‘21GAQ-1271’ in 2021. 32 of the resulting F1 seed were sown. ‘PTC8506V’ was discovered and selected as a single plant from this F1 seed and was subsequently propagated clonally in Hillsboro, OR. ‘PTC8506V’ was asexually reproduced by apical and sub-apical semi-herbaceous stem cuttings in Hillsboro, Oregon for eight cycles. Asexual reproduction has demonstrated that ‘PTC8506V’ reproduces true to type with all of the characteristics, as herein described, and firmly fixed and retained through successive generations of asexual propagation.

The new variety is named ‘PTC8506V’ and is characterized as having elevated levels of THCV. In comparison to its parents, ‘PTC8506V’ has higher THCV (relative to either parent), and higher THC relative to the pollen donor plant. ‘PTC8506V’ can additionally be distinguished from either parent at least based on the characteristics provided in Table 1.

TABLE 1

Comparison to Parent Varieties.

Trichome Aroma Aroma Growth Inter- Maturity

Variety Coverage Strength Type Habit node Time

‘21GAQ- Heavy Strong Sweet Upright Moder- 8-9

1271’ cookies, ately weeks

earth, long

garlic

‘21VLP5-1- Low Moder- Grass, Moder- Long 10-12

102’ ately earth, ately weeks

weak citrus wide

‘PTC8506V’ Moderate Moder- Pine, Upright Moder- 8-10

ately earth, ately weeks

strong fuel, long

and a

slight

fruiti-

ness

BRIEF DESCRIPTION OF THE PHOTOGRAPH

FIG. 1 is a photograph of a flowering plant of ‘PTC8506V’ that is approximately 83 days old, growing in Hillsboro, Oregon.

DETAILED BOTANICAL DESCRIPTION

The following is a detailed description of the new Cannabis variety ‘PTC8506V’. Unless otherwise indicated, all color designations provided herein are hex color codes.

All breeding, cultivation, and chemotype analyses were conducted in Hillsboro, Oregon.

Cannabis plants having elevated THCV or THC levels were sourced and obtained, and crosses were conducted to produce new varieties to further elevate THCV and THC levels. The plants were crossed to produce new varieties having elevated THCV and THC levels. Plant variety ‘PTC8506V’ was selected and asexually reproduced.

Table 2 describes the general characteristics of ‘PTC8506V’ at 55 days after lighting was changed to 12 hours on/12 hours off.

TABLE 2

Plant life forms An herbaceous plant (herb)

Plant growth habit An upright, tap-rooted annual

plant, forming fibrous

roots when

asexually propagated

Plant origin A controlled cross between

pollen acceptor

(‘21GAQ-1271’) and pollen donor

plant (‘21VLP5-1-102).

Plant propagation Asexually propagated by apical

and sub-apical semi-

herbaceous stem cuttings

Propagation Ease Easy

Plant shape Simple erect

Height 117 cm to 121 cm

Width 87 cm to 101 cm

Time to harvest 10-12 weeks

Resistance to pests or diseases Unknown

GMO? No

Table 3 describes the leaf/foliage of ‘PCT8506V’. Color codes are hex color codes.

TABLE 3

Leaf Arrangement Alternate

Leaflet Type Palmately compound

Leaflet Structure Serrated margins, lightly acicular

to lanceolate leaflets,

tapering to an acuminate apex

Leaflet Margins Jaggedly serrate with each tooth

apex angled toward

leaflet apex

Leaf hairs Present on both upper and

lower surfaces

Leaf length with petiole at 18.5 cm to 23 cm

maturity

Petiole length at maturity 6 cm to 9.5 cm

Petiole diameter at maturity 1.9 mm to 2.5 mm

Petiole color #6B4247

Anthocyanin color and intensity #F9CCC5. Medium intensity

in Petioles in vegetative stage;

stronger in flowering stage

Stipule length at maturity 6.2 mm to 6.8 mm

Stipule shape Linear with slight curvaceous

growth

Stipule color #3C4D03

No. of leaflets 3 to 7

Middle largest (longest) leaflet 12 cm to 17.5 cm

length

Middle largest (longest) leaflet 2.5 cm to 3.1 cm

width

Middle largest (longest) leaflet 3.8 to 4.8

length/width ratio

No. teeth of middle leaflet 28 to 34

Leaf (upper side-adaxial) color #35530A

Leaf (lower side-abaxial) color #35530A

Leaf glossiness Matte

Vein/midrib shape Obliquely continuous

throughout leaflet

Vein/midrib color #E6DAA6

Aroma Earthy notes of pine, fuel, and

a slight fruitiness

Table 4 describes the stem of ‘PTC8506V’.

TABLE 4

Stem shape Hollow, ribbed, texture

Stem length 117 cm to 121 cm

Stem strength Strong and upright

Stem diameter at base 13.6 mm to 14.5 mm

Stem color #373E02

Depth of main stem ribs/grooves Shallow

Internode length 3 cm to 9.5 cm

Table 5 describes the inflorescence of ‘PTC8506V’.

TABLE 5

Inflorescence type Elongated compound cymes

Flowering (blooming) habit Natural flowering season is

Autumn in Hillsboro, Oregon.

Proportion of female plants 100% pistillate

Inflorescence position Terminal and axillary

Number of inflorescences 100 to 150

produced per plant

Flower arrangement Cymose

Length of inflorescences 3 cm to 3.8 cm

Numbers of flowers per plant Hundreds to thousands flowers per

plant, multitudinous, congested

with high concentrations on

bushier wide inflorescence

Flower shape Urceolate

Flower (individual pistillate) 5.1 mm to 5.3 mm

length

Flower (individual pistillate) 2 mm

depth

Flower (compound cyme) 5.5 cm to 8 cm

diameter

Corolla No defined corolla

Corolla color N/A

Bract shape Urceolate

Bract color #4B5D16

Typical bract numbers 3 to 200 bracts per inflorescence.

Bract length 3 mm to 5mm

Bract width 2.3 mm to 2.7 mm

Bract apex and base description Bracts have a rounded base and

caudate apex.

Bract Texture Bract texture is smooth and

covered with sticky trichomes.

Bracteole number, shape, length, Bracteoles are not present

width, apex, margin, base

descriptors, texture, and color

Calyx shape No defined calyx

Calyx color N/A

Pistil number per flower One

Pistil color Pistil consists of the stigma, style,

and ovary and cannot be

given a single color.

Stigma shape Acute

Number of stigmas per flower Two

Stigma length 4.6 mm to 6.5 mm

Stigma color #373E02

Trichome shape Capitate-stalked glandular

Trichome color #F1E7E2

Terminal inflorescence shape Oblong

Terminal inflorescence color #B7E1A1

Terminal inflorescence length 12.5 cm to 14 cm

Terminal inflorescence width 5 cm to 6.5 cm

Pedicel Absent

Peduncle length 6.8 mm

Peduncle diameter 4.1 mm

Peduncle texture Lightly ribbed

Peduncle color #9BB53C

Staminate shape No staminate flowers produced

naturally; however, male

flower (staminate) can be

induced with stress or ethylene-

blocking compounds

Sepal color N/A

Pollen description N/A

Petal description Apetalous

Table 6 describes other characteristics of ‘PTC8506V’

TABLE 6

Time period and condition 8 to 10 weeks

of flowering/blooming

Proportion of hermaphrodite Lower (<5%) in clones

plants

Hardiness of plant Hardy to tolerate of warm

and dry conditions

without showing stress.

Not tolerant to soil

oversaturated with water

for long periods

Chemical analyses were conducted using High Performance Liquid Chromatography to detect the cannabinoids described herein. Table 7 describes a chemotype analysis of ‘PTC8506V’, which was chemotyped by taking a sample from the main cola at day 56 after initiation of the 12:12 light cycle, which initiates flowering. Trichomes were 70% cloudy and 5% amber, indicative of full maturity at sampling.

TABLE 7

Cannabinoid and other chemicals % wt

Total THC 4.613

Total THCV 11.067

Total CBG 0.047

Total CBD <LOD

Total CBC <LOD

Total CBGV <LOD

Total CBDV <LOD

alpha-Pinene 0.057

Camphene 0.011

beta-Myrcene 1.557

beta-Pinene 0.098

delta-3-Carene <LOQ

alpha-terpinene <LOQ

D-Limonene 0.211

beta-Ocimene 0.275

p-Cymene <LOQ

Eucalyptol <LOQ

gamma-Terpinene <LOQ

Terpinolene <LOQ

Linalool 0.003

Isopulegol <LOQ

Geraniol <LOQ

beta-Caryophyllene 0.05

alpha-Humulene 0.0227

Nerolidol 1 <LOQ

Nerolidol 2 0.011

Guaiol <LOQ

Caryophyllene Oxide 0.011

alpha-Bisabolol 0.026

Total Terpenes 2.315