Cannabis Plant Named ‘PCT11204V’

Genus/species: Cannabis sativa.

Variety denomination: ‘PCT11204V’.

BACKGROUND OF THE INVENTION

Cannabis plants contain over a hundred known cannabinoids, which bind to endogenous endocannabinoid receptors. Varinolic cannabinoids, known as varins, are a type of cannabinoid compound having three carbon atoms in their alkyl side chain instead of the five carbon atom alkyl side chains more commonly associated with cannabinoids. Two such varins are tetrahydrocannabivarin (THCV) and cannabidivarin (CBDV), which are homologues of tetrahydrocannabinol (THC) and cannabidiol (CBD), respectively. Each varin has a unique pharmacological profile and distinct molecular targets.

THCV and CBDV have potential benefits across a broad set of applications. Cannabis plants or extracts with high THCV levels, for example, can be used as an agent for anticonvulsant activity, obesity-associated glucose intolerance, appetite suppression, anxiety management for PTSD, diabetic neuropathy, and major neuropathic and pain related pathologies. Another THCV application is that of an appetite suppressing compound. CBDV has been shown to have anti-epileptic and anticonvulsant activity.

Research and development as well as the sale of varin products has been limited due to low commonly occurring levels of varins in Cannabis flower. The ability to produce Cannabis with high varin levels will create a platform for a new Cannabinoid category with differentiated, high margin products in both medical and recreational markets. The present invention solves these problems by providing a novel Cannabis plant having high varin levels, which is referred to by the variety name ‘PCT11204V’.

BRIEF SUMMARY OF THE INVENTION

The new variety of Cannabis sativa was created by sourcing Cannabis plants that produce THCV. All breeding, cultivation, and chemotype analyses were conducted by a contract research organization under contract for the Applicant. Crosses were then conducted to elevate further THCV and other varins. The plants are crossed to produce new varieties that are selected to further elevate THCV levels. Progeny are chemically analyzed to select those having elevated THCV levels, and then either (1) backcrossed to the high-yielding THCV parent to ensure additional progeny can be selected for increased THCV production, or (2) selfed to fix genetic loci associated with THCV levels to ensure additional progeny can be selected for increased THCV production. Marker-assisted selection may be used further to identify candidate progeny expected to have elevated THCV levels for subsequent crosses to ensure selection of progeny having the highest THCV levels. All chemical analyses were conducted using High Performance Liquid Chromatography to detect the cannabinoids described herein. The present invention is a selection resulting from the cross of a high THCV pollen receiver plant (“20VLDY-1002-3”) and a high THC pollen donor plant (“20TP1B-1008-1”). Parent cultivar 20VLDY-1002-3 is a late maturing (>12 weeks) cultivar with long internode lengths, flowers with low trichome density, a THCV content of 4-6%, and a THC content of 4-6%. Parent cultivar 20TP1B-1008-1 is similar to typical cultivated cultivars, with a maturity of 9 weeks from flower initiation, moderate internode lengths, a THC content of 21%, and a THCV content of 1%. Ninety seeds of variety “THC-Victory” were planted Aug 17, 2020. From this population, one plant designated “20VLDY-1002-3” of THC content 11.9% and THCV content 13.96% was selected and cloned for future crosses. 24 seeds of variety “Guicy Banger” were planted Jun. 19, 2020. From this population, one plant designated “20TP1B-1008-1” of THC content 20.66% and THCV content 1.16% was selected and cloned for future crosses. A clone of 20TP1B-1008-1 was crossed onto a clone of 20VLDY-1002-3 in the spring of 2021. 144 of the resulting F1 seeds were sown May 14, 2021. The present invention was discovered and selected as a single plant from this F1 seed and was subsequently propagated clonally at a contract research organization under contract for the Applicant. The selection was subsequently evaluated for five cycles at a contract research organization under contract for the Applicant. Individual plants were propagated in an indoor facility with supplemental lighting. Plants are transferred to a mixed-light greenhouse once established. Asexual reproduction of the new variety by stem cutting propagation since 2021 at the contract research organization under contract for the Applicant, and has demonstrated that the new variety reproduces true to type with all the characteristics, as herein described and are, firmly fixed and retained through successive generations of asexual propagation.

The new variety is named ‘PCT11204V’ and is characterized as having elevated varin levels.

BRIEF DESCRIPTION OF THE PHOTOGRAPH

FIG. 1 is a color image of the entire plant of a 89 day old flowering plant of the variety ‘PCT11204V’ taken at a contract research organization under contract for the Applicant.

DETAILED BOTANICAL DESCRIPTION

The following is a detailed description of the new Cannabis variety known as ‘PCT11204V’.

All breeding, cultivation, and chemotype analyses were conducted by a contract research organization under contract for the Applicant.

Cannabis plants having elevated THCV levels were sourced and obtained, and crosses were conducted to further elevate THCV levels. The plants were crossed to produce new varieties that were selected to further elevate THCV levels. Plant variety ‘PCT11204V’ was selected and asexually reproduced.

Table 1 describes the general characteristics of ‘PCT11204V’ at 60 days after lighting was changed to a 12/12 light cycle. Color codes are derived from The Royal Horticultural Society (R.H.S.) Color Chart.

TABLE 1

Plant life forms An herbaceous plant (herb)

Plant growth habit An upright, tap-rooted annual plant,

forming fibrous roots when asexually

propagated

Plant origin A controlled cross between female parent

20VLDY-1002-3 (unpatented) and male

parent 20TP1B-1008-1 (unpatented)

Plant propagation Asexually propagated by replanting of

apical and sub-apical semi-heraceous

stem cuttings

Propagation Ease Easy

Height 1-1.3 m

Width 0.7-0.9 m

Plant shape Simple erect

Time to harvest 10-12 weeks

Resistance to pests Unknown

or diseases

GMO? No

Table 2 describes the leaf/foliage of ‘PCT11204V’.

TABLE 2

Leaf Arrangement Alternate

Leaflet Type Palmately compound

Leaflet Structure Serrated margins, lightly acicular to

lanceolate leaflets, tapering to an

acuminate apex

Leaflet Margins and Base Margins are jaggedly serrate with each

tooth apex angled toward leaflet apex; and

base is attenuate.

Leaflet hairs Present on both upper and lower surfaces

Leaf length with petiole 14-22 cm

at maturity

Petiole length and Length is 3.6-6.5 cm and diameter is

diameter at maturity 1-2 mm

Petiole color 45C

Anthocyanin color and Color is 47C and intensity in Petioles is

intensity in Petioles medium in vegetative stage; strong in

flowering stage

Stipule length and width Length is 0.3-0.7 cm width is <1 mm

at maturity

Stipule shape Linear with slight curvaceous growth

Stipule color 149C

No. of leaflets 3-7

Middle largest (longest) 11.5-15.2 cm

leaflet length

Middle largest (longest) 2.5-3.2 cm

leaflet width

Middle largest (longest) 4.5-4.8

leaflet length/width ratio

No. teeth of middle leaflet 20-26

Leaflet (upper side-adaxial) 140A

color

Leaflet (lower side-abaxial) 135D

color

Leaflet glossiness Slightly glossy on the upper surface

Vein/midrib shape Obliquely continuous throughout leaflet

Vein/midrib color 149D

Venation pattern Pinnately veined leaflets

Aroma Pleasantly pungent aromatic, strong,

peculiar smell with a hint of cleaning

solution scent

Table 3 describes the stem of ‘PCT11204V’.

TABLE 3

Stem description Hollow and ribbed

Stem diameter at base 1.5 cm

Stem color 142A

Stem length 90-110 cm

Stem texture Smooth with shallow grooves

Stem strength Strong and upright

Depth of main stem ribs/grooves Shallow

Internode length 2.5-10 cm

Table 4 describes the inflorescence of ‘PCT11204V’.

TABLE 4

Inflorescence type Elongated compound cymes with the

natural flowering season being Autumn

Proportion of female plants 100% pistillate

Inflorescence position Terminal and axillary

Inflorescence number per 100-150

plant

Inflorescence length 2-10 cm

Flower arrangement Cymose

Numbers of flowers per Hundreds to thousands flowers per plant,

plant multitudinous, congested with high

concentrations on bushier wide

inflorescence

Flower shape Urceolate

Flower (individual 5-6 mm

pistillate) length

Flower (compound 8.5-10.0 cm

cyme) diameter

Typical and observed 2-4 mm

flower depth

Corolla No defined corolla

Corolla color N/A

Bract shape Urceolate

Bract color upper surface 135C

Bract color lower surface 135C

Bract number 3-200 bracts per inflorescence

Bract length 3-5 mm

Bract width 2-3 mm

Bract apex Caudate apex

Bract base descriptors Rounded base

Bract texture Smooth and covered with sticky trichomes

Calyx shape No defined calyx

Calyx color N/A

Stigma shape Acute

Stigma length 5-7 mm

Stigma width <1 mm

Stigma color 140B

Number of stigmas per 2

flower

Trichome shape Capitate-stalked glandular

Trichome color NN155B

Terminal bud shape Oblong

Terminal inflorescence 18-24 cm

length

Terminal inflorescence 7-10 cm

diameter

Terminal bud color 140A, 140B, and 140C

Pedicel Absent

Peduncle length 1-8 mm

Peduncle diameter 2-3 mm

Peduncle texture Lightly ribbed

Peduncle color 145A

Staminate shape No staminate flowers produced naturally;

however, male flower (staminate) can be

induced with stress or ethylene-blocking

compounds

Sepal color NA

Pollen description NA

Seed shape Striped, smooth and globular

Seed size 1.6 - 2.3 mm

Petal description Apetalous

Pistil number per flower one

Pistil length 10-12 mm

Bracteole Not present

Table 5 describes other characteristics of ‘PCT11204V’.

TABLE 5

Time period of flowering/ 10-12 weeks

blooming

Proportion of hermaphrodite Lower (<5%) in clones

plants

Hardiness of plant Hardy to tolerate of warm and dry

conditions without showing stress. Not

tolerant to soil oversaturated with water for

long periods

Chemical analyses were conducted using High Performance Liquid Chromatography to detect the cannabinoids described herein. Table 6 describes a chemotype and terpene analysis of ‘PCT11204V’, which was chemotyped by taking a sample from the main cola at day 83 after initiation of the 12:12 light cycle, which initiates flowering. Trichomes were 65% cloudy and 1% amber, indicative of full maturity at sampling.

TABLE 6

Cannabinoid data for ‘PCT11204V’ (day 83 after flowering onset).

Cannabinoid % wt

Tetrahydrocannabivarinic acid 15.6

(THCVA)

THCV 0.28

Total THCV 140

Cannabidivarinic acid (CBDVA) 0.1

CBDV <LOD

Total CBDV 0.09

Total THCV + Total CBDV (= Total 14.0

Varins)

Total THC + Total CBD (= Total 11.9

Cannabinoids)

Total Varins/Total Cannabinoids 1.18

Tetrahydrocannabinolic acid (THCA) 13.3

Delta-9-tetrahydrocannabinol (D9-THC) 0.2

Total THC 11.9

Cannabidiolic acid (CBDA) <LOD

CBD <LOD

Total CBD <LOD

THC:CBD ratio Undefined value due to null

value in denominator

beta-myrcene 0.9-2.8%

beta-caryophyllene 0.3-0.5%

ocimene 0.3-0.8%

bisabolol 0.08-0.14%

alpha-humulene 0.08-0.15%

“<LOD” refers to an undetectable analyte that is below the limit of detection.